Depends on the target and the setup, ideally if you have 4 NICs you should be using at least two different VLANs, and since you have 4 NICs (I assume for iSCSI only) you should use jumbo frames.
Jumbo frames should only be used if your CPU can't keep up with the load of 4 NICs otherwise it does add some latency to iSCSI.
How much latency are we talking about?
I'm looking into gigabit networks at for both home & work and while home isn't so much an issue, work could be as I'm in the planning stages of setting up a gigabit network to allow some iSCSI NAS/SAN devices to back a bunch of VMware servers that we're due to get for our next refresh.